Non-intrusive dimensions off mRNA decay suggests translation initiation since the major determinant regarding mRNA balances
The brand new cytoplasmic variety out of mRNAs is exactly controlled thanks to a balance off production and you can destruction. While the brand new power over mRNA synthesis as a consequence of transcription might have been better distinguisheded, quicker is known concerning controls of mRNA turnover, and you will an opinion model outlining the broad differences in mRNA decay prices stays challenging. Here, we merge non-invasive transcriptome-wider mRNA production and you may balances measurements with selective and you will intense perturbations to exhibit that mRNA degradation try firmly coupled on the regulation regarding interpretation, and therefore a competition ranging from interpretation initiation and you can mRNA decay -but not codon optimality otherwise elongation- ‘s the big determinant away from mRNA balances within the fungus. Our very own subdued measurements in addition to reveal an excellent reic transcriptome having the typical mRNA half of-life of merely cuatro.8 minute – far reduced than previously consider.More